| Size | Price | Stock | Qty |
|---|---|---|---|
| 500mg |
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| 1g |
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| Other Sizes |
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| ln Vitro |
At 7 weeks, homozygous KO and KI mice on a standard diet had lower blood thiamine levels than WT mice (0.796±0.259 μM), at 0.058±0.051 and 0.126±0.092 μM, respectively. Days 5 and 14 saw a significant decrease in blood thiamine concentrations to 0.010 in WT and homozygous KO and KI mice fed a thiamine-restricted diet (thiamine: 0.60 mg/100 g of chow). 0.010±0.006 and ±0.009 μM. mice of WT (0.609±0.288 μM). In WT mice given a typical diet, the brain homogenates had a thiamine concentration of 3.81±2.18 nmol/g wet weight, while KO and KI brain homogenates had thiamine values of 1.33±0.96 and 2.16±1.55 nmol/g wet weight, respectively. Notably, following 5 days (0.95±0.72 nmol/g wet weight) and 14 days (1.11±0.24) of eating a thiamine-restricted diet (thiamine: 0.60 mg/100 g food), the KO and KI were reduced. In comparison to WT (3.65 ± 1.02 nmol/g wet weight), thiamine concentrations in mouse brain homogenates gradually drop before the mice exhibit illness symptoms [2].
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| ln Vivo |
On a typical diet containing 1.71 mg/100 g of thiamine, WT, homozygous and heterozygous KO, and KI mice survived for over six months without exhibiting any signs of disease. After being fed a diet restricted in thiamine (thiamine: 0.60 mg/100 g chow), homozygous KO and KI mice showed signs of paralysis, weight loss, and immobility, respectively. These mice died after 12 and 30 days, respectively. Likewise, within 14 and 18 days, respectively, homozygous KO and KI mice that were fed a diet restricted in thiamine and had a reduced thiamine percentage (thiamine: 0.27 mg/100 g of food) perished. Nevertheless, mice administered a thiamine-restricted diet (thiamine: 0.60 mg or 0.27 mg/100g of chow) in both WT and heterozygous KO and KI groups survived for over 6 months without exhibiting any signs of sickness [2].
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| References |
[1]. Kenneth Osiezagha, et al.Thiamine Deficiency and Delirium. Innov Clin Neurosci. 2013 Apr; 10(4): 26-32.
[2]. Kaoru Suzuki, et al. High-dose thiamine prevents brain lesions and prolongs survival ofSlc19a3-deficient mice. PLoS One. 2017; 12(6): e0180279 |
| Molecular Formula |
C12H17CLN4OS
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|---|---|
| Molecular Weight |
300.8076
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| CAS # |
59-43-8
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| Related CAS # |
Thiamine nitrate;532-43-4;Thiamine hydrochloride;67-03-8
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| Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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| SMILES |
[Cl-].S1C([H])=[N+](C([H])([H])C2=C([H])N=C(C([H])([H])[H])N=C2N([H])[H])C(C([H])([H])[H])=C1C([H])([H])C([H])([H])O[H]
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ≥ 100 mg/mL (~332.44 mM)
DMSO : ~1 mg/mL (~3.32 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 100 mg/mL (332.44 mM) (saturation unknown) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.3244 mL | 16.6218 mL | 33.2436 mL | |
| 5 mM | 0.6649 mL | 3.3244 mL | 6.6487 mL | |
| 10 mM | 0.3324 mL | 1.6622 mL | 3.3244 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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