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Purity: ≥98%
Thiazovivin (TZV) is a novel, selective, and cell-permeable small molecule ROCK (Rho-associated kinase) inhibitor with an IC50 of 0.5 μM in a cell-free assay. It can protect human embryonic stem cells (hESC). Thiazovivin promotes hESC survival after single-cell dissociation and improves stemness maintenance of embryo-derived stem-like cells under chemically defined culture conditions in cattle. Thiazovivin directly targets ROCK and increases expression of pluripotency factors. The process using thiazovivin could be easier, faster and more cost effective than transgene integration into somatic cells.
| ln Vitro |
One ROCK inhibitor is thiazovivin. Thiazovivin (2 μM) preserves human embryonic stem cells (hESC) via blocking ROCK activity. After separation, thiazovivin dramatically prolongs hESCs' lifespan while preserving their pluripotency. Thiazovivin improves integrin signaling mediated by cell-ECM adhesion. Additionally, thiazovivin stabilizes E-cadherin upon cell dissociation to prevent hESCs from dying in the absence of extracellular matrix [1]. Thiazovivin promotes primary colony development and cell attachment of bovine embryonic-derived stem cell-like cells (eSLC) on feeder layers. Thiazovivin promotes the growth of eSLC cultures during passage and augments putative colony growth. Moreover, in bovine eSLC, thiazovivin increases the expression of genes unique to the ectodermal lineage [2].
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| References |
[1]. Xu Y, et al. Revealing a core signaling regulatory mechanism for pluripotent stem cell survival and self-renewal by small molecules. Proc Natl Acad Sci U S A. 2010 May 4;107(18):8129-34.
[2]. Park S, et al. Thiazovivin, a Rho kinase inhibitor, improves stemness maintenance of embryo-derived stem-like cells under chemically defined culture conditions in cattle. Anim Reprod Sci. 2015 Oct;161:47-57 |
| Molecular Formula |
C15H13N5OS
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| Molecular Weight |
311.36
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| CAS # |
1226056-71-8
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| SMILES |
O=C(C1=CSC(NC2=NC=NC=C2)=N1)NCC3=CC=CC=C3
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
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| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (8.03 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (8.03 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. View More
Solubility in Formulation 3: 30% PEG400+0.5% Tween80+5% propylene glycol:30 mg/mL |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.2117 mL | 16.0586 mL | 32.1172 mL | |
| 5 mM | 0.6423 mL | 3.2117 mL | 6.4234 mL | |
| 10 mM | 0.3212 mL | 1.6059 mL | 3.2117 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
 Novel synthetic small molecules dramatically increase hESC survival after single cell dissociation by enhancing cell-ECM mediated integrin activity.Proc Natl Acad Sci U S A.2010 May 4;107(18):8129-34. |
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 Tzv stabilizes E-cadherin after cell dissociation to protect hESCs from death under ECM-free conditions.Proc Natl Acad Sci U S A.2010 May 4;107(18):8129-34. |
 Tzv is a novel ROCK inhibitor and Rho-ROCK axis regulates cell-ECM and cell-cell adhesion.Proc Natl Acad Sci U S A.2010 May 4;107(18):8129-34. |
 Cell-cell interaction regulates cell-ECM interaction and contributes to survival and self-renewal of hESCs.Proc Natl Acad Sci U S A.2010 May 4;107(18):8129-34. |
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 hESCs maintained in mESCs culture condition have better survival and are dependent on different cell adhesion signaling for survival and self-renewal.Proc Natl Acad Sci U S A.2010 May 4;107(18):8129-34. |
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