| Size | Price | Stock | Qty |
|---|---|---|---|
| 5g |
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| Other Sizes |
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Purity: ≥98%
| ln Vitro |
Thioflavin T (ThT) is a benzothiazole dye that is frequently used to detect amyloid fibrils both in vitro and ex vivo. It exhibits heightened fluorescence upon binding to amyloid fibrils. Thioflavin T is present in aqueous solutions as micelles at concentrations of around 10–20 μM, which are frequently employed to monitor fibrils using fluorimetry. After measuring specific conductivity changes at various thioflavin T concentrations, 4.0±0.5 μM was determined to be the crucial micelle concentration. The generation of micelles affects thioflavin T's fluorescence excitation and emission as well. Using atomic force microscopy, thioflavin T micelles with a diameter of 3 nm were directly detected, and along the fiber length, thioflavin T micelle production with unique fibrils was noted. An increase in the number of micelles bound along the fiber length is shown by rising thioflavin T concentrations above the critical micelle concentration. Atomic force microscopy shows that thioflavin T micelles are broken down at low pH levels and that the fluorescence increase that results from thioflavin T binding to amyloid fibrils also declines many times below 3. Amyloid fibrils are bound by thioflavin T micelles, which increases fluorescence emission [1].
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| References | |
| Additional Infomation |
Thioflavine T is an organic chloride salt having 2-[4-(dimethylamino)phenyl]-3,6-dimethyl-1,3-benzothiazol-3-ium as the counterion. It is widely used to visualise and quantify the presence of amyloids, both in vitro and in vivo. It has a role as a fluorochrome, a histological dye and a geroprotector. It contains a thioflavin T cation.
|
| Molecular Formula |
C17H19CLN2S
|
|---|---|
| Molecular Weight |
318.8642
|
| Exact Mass |
318.095
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| CAS # |
2390-54-7
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| PubChem CID |
16953
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| Appearance |
Yellow to orange solid powder
|
| Melting Point |
212ºC
|
| LogP |
0.771
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| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
2
|
| Heavy Atom Count |
21
|
| Complexity |
325
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
JADVWWSKYZXRGX-UHFFFAOYSA-M
|
| InChi Code |
InChI=1S/C17H19N2S.ClH/c1-12-5-10-15-16(11-12)20-17(19(15)4)13-6-8-14(9-7-13)18(2)3;/h5-11H,1-4H3;1H/q+1;/p-1
|
| Chemical Name |
4-(3,6-dimethyl-1,3-benzothiazol-3-ium-2-yl)-N,N-dimethylaniline;chloride
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~16.67 mg/mL (~52.28 mM)
H2O : ~5 mg/mL (~15.68 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.67 mg/mL (5.24 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 16.7 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.1362 mL | 15.6809 mL | 31.3617 mL | |
| 5 mM | 0.6272 mL | 3.1362 mL | 6.2723 mL | |
| 10 mM | 0.3136 mL | 1.5681 mL | 3.1362 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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