| Size | Price | Stock | Qty |
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| 500mg |
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| 5g |
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| 25g |
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Purity: ≥98%
Thymidine (also known as deoxythymidine; other names deoxyribosylthymine, thymine deoxyriboside) is a pyrimidine deoxynucleoside used as a cell synchronizing agent. The nucleoside T in double-stranded DNA is deoxythymidine, and it pairs with deoxyadenosine (A). It synchronizes the cells in the G1/early S phase, which is useful in cell biology.
| Targets |
Microbial Metabolite; DNA Synthesis; Human Endogenous Metabolite
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|---|---|
| ln Vitro |
Thymidine (NSC 21548), a pyrimidine nucleoside that pairs with adenine during DNA synthesis. It is made up of the pyrimidine base thymine attached to the sugar deoxyribose.[1]
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| ln Vivo |
The loss of CD4+Vβ8+ and CD8+Vβ8+ T cells induced by thymidine and Tomudex is totally restored by thymidine (500 mg/kg; i.p.; twice daily) [3].
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| Cell Assay |
72 hours following the infection of shRNA lentiviruses, 4 mM thymidine is applied to MDA-MB-231 cells for 18 hours. At 0, 6, and 18 hours, release and harvest cells. Use FACS analysis to ascertain the cell cycle distribution.
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| Animal Protocol |
Animal/Disease Models: 8-12 weeks balb/c (Bagg ALBino) mouse [3]
Doses: 500 mg/kg Route of Administration: intraperitoneal (ip) injection; twice (two times) daily Experimental Results: After injection of SEB, methotrexate and Tomudex-induced Vβ8+ T were completely eliminated Cell loss. |
| References |
[1]. Chen G, et al. Cell Synchronization by Double Thymidine Block. Bio Protoc. 2018 Sep 5;8(17).
[2]. FIRKET H, et al. Autoradiographic visualization of synthesis of deoxyribonucleic acid in tissue culture with tritium-labelled thymidine. Nature. 1958 Jan 24;181(4604):274-5.FIRKET H, et al. Autoradiographic visualization of synthesis of deoxyribonucleic acid in tissue culture with tritium-labelled thymidine. Nature. 1958 Jan 24;181(4604):274-5. [3]. Izeradjene K, et al. Inhibition of thymidine synthesis by folate analogues induces a Fas-Fas ligand-independentdeletion of superantigen-reactive peripheral T cells. Int Immunol. 2001 Jan;13(1):85-93. |
| Molecular Formula |
C10H14N2O5
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|---|---|
| Molecular Weight |
242.2286
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| Exact Mass |
242.09
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| Elemental Analysis |
C, 49.58; H, 5.83; N, 11.56; O, 33.02
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| CAS # |
50-89-5
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| Related CAS # |
33430-62-5 (Thymidine-5-monophosphate disodium salt)
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| Appearance |
Solid powder
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| SMILES |
CC1=CN(C(=O)NC1=O)[C@H]2C[C@@H]([C@H](O2)CO)O
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| InChi Key |
IQFYYKKMVGJFEH-XLPZGREQSA-N
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| InChi Code |
InChI=1S/C10H14N2O5/c1-5-3-12(10(16)11-9(5)15)8-2-6(14)7(4-13)17-8/h3,6-8,13-14H,2,4H2,1H3,(H,11,15,16)/t6-,7+,8+/m0/s1
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| Chemical Name |
1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-methylpyrimidine-2,4-dione
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| Synonyms |
DThyd; Deoxyribothymidine; Deoxythymidine; NSC 21548; NSC-21548; NSC21548; Thymidin; AI3-52267; AI3 52267; AI352267
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| HS Tariff Code |
2934.99.03.00
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 48~50 mg/mL (198.2~206.4 mM)
H2O: ~33.3 mg/mL (~137.6 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 20 mg/mL (82.57 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication (<60°C).
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.1283 mL | 20.6415 mL | 41.2831 mL | |
| 5 mM | 0.8257 mL | 4.1283 mL | 8.2566 mL | |
| 10 mM | 0.4128 mL | 2.0642 mL | 4.1283 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
| NCT Number | Recruitment | interventions | Conditions | Sponsor/Collaborators | Start Date | Phases |
| NCT03639701 | Active Recruiting |
Drug: Thymidine | Thymidine Kinase 2 Deficiency | Columbia University | May 16, 2017 | Phase 1 Phase 2 |
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