| Size | Price | Stock | Qty |
|---|---|---|---|
| 10mg |
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| Other Sizes |
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Purity: ≥98%
TLCK hydrochloride (-alpha-Tosyl-L-lysine chloromethyl ketone) is a novel potent trypsin like protease inhibitor and a blocker of the LPS- or cytokine-induced activation of nuclear factor κB (NF-κB). sensitizes HeLa cells to Fas-mediated cell death.
| ln Vitro |
On IFN-γ activity, N-α-Tosyl-L-lysine chloromethyl ketone has an inhibiting effect. It was investigated how TLCK affected HeLa cells' IFN-γ sensitivity in preventing Fas-mediated cell death. Three effectors—anti-Fas, TLCK, and IFN-γ—were simultaneously detected when anti-Fas was present at a lower quantity (10 ng/mL). Cell viability somewhat decreased when TLCK itself was at a 50 μM concentration. Cell viability was found to diminish in a dose-dependent manner over 50 μM. Cell viability is modestly decreased by IFN-γ alone. When anti-Fas (10 ng/mL) was added, cell viability was slightly reduced; however, when TLCK was present, cell viability was more than additively enhanced, especially between 50 and 100 μM. The inclusion of IFN-γ and anti-Fas resulted in a roughly 46% reduction in cell viability. Moreover, the addition of increasing quantities of TLCK (25 μM, etc.) exacerbates the reduction in cell viability [1].
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| References | |
| Additional Infomation |
An inhibitor of SERINE ENDOPEPTIDASES. Acts as an alkylating agent and is known to interfere with the translation process.
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| Molecular Formula |
C14H22CL2N2O3S
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|---|---|
| Molecular Weight |
333.85408
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| Exact Mass |
368.072
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| CAS # |
4272-74-6
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| Related CAS # |
2364-87-6;4272-74-6 (HCl);
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| PubChem CID |
73093
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| Appearance |
Typically exists as solid at room temperature
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| Boiling Point |
509.8ºC at 760 mmHg
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| Melting Point |
-165ºC (dec.)
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| Flash Point |
262.1ºC
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| LogP |
4.552
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
9
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| Heavy Atom Count |
22
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| Complexity |
414
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| Defined Atom Stereocenter Count |
1
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| SMILES |
O=S(C1=CC=C(C)C=C1)(N[C@H](C(CCl)=O)CCCCN)=O.Cl
|
| InChi Key |
YFCUZWYIPBUQBD-ZOWNYOTGSA-N
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| InChi Code |
InChI=1S/C14H21ClN2O3S.ClH/c1-11-5-7-12(8-6-11)21(19,20)17-13(14(18)10-15)4-2-3-9-16/h5-8,13,17H,2-4,9-10,16H2,1H31H/t13-/m0./s1
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| Chemical Name |
(S)-N-(7-amino-1-chloro-2-oxoheptan-3-yl)-4-methylbenzenesulfonamide hydrochloride
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| Synonyms |
TLCK hydrochloride TLCK HCl Tosyllysine Chloromethyl Ketone hydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~250 mg/mL (~676.94 mM)
H2O : ~100 mg/mL (~270.78 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (5.63 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (5.63 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (5.63 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9954 mL | 14.9768 mL | 29.9536 mL | |
| 5 mM | 0.5991 mL | 2.9954 mL | 5.9907 mL | |
| 10 mM | 0.2995 mL | 1.4977 mL | 2.9954 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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