| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg |
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| Other Sizes |
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| ln Vitro |
By triggering the ERK1/2 signaling pathway, trametenolic acid (1 mg/mL; 15–30 minutes) causes the overexpression of autophagy-related proteins (Beclin-1, Atg12, and LC3) in B16-F1 cells [1]. Through a twofold synergistic mechanism, trimethenoic acid (1 mg/mL; 24 hours; B16-F1 cells) has antimelanogenic actions [1]. Melanin synthesis was boosted by roughly 20% and 40% in cells transfected with siRNA against mTOR and Atg5, respectively, by trimethenoic acid (0.1-4 mg/mL; 52 hours; B16-F1 cells) [1].
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|---|---|
| Cell Assay |
Western Blot Analysis[1]
Cell Types: B16-F1 Cell Tested Concentrations: 1 mg/mL Incubation Duration: 15 and 30 minutes Experimental Results: Increased expression levels of phosphorylated p38, phosphorylated ERK1/2, Beclin-1, Atg12 and LC3 respectively About 46%, 10%, 48%, 126% and 49%. Western Blot Analysis[1] Cell Types: B16-F1 Cell Tested Concentrations: 1 mg/mL Incubation Duration: 24 hrs (hours) Experimental Results: Increased expression of ERK1/2 and p-ERK1/2, diminished MITF, tyrosinase and TRP 1 levels. |
| References | |
| Additional Infomation |
Trametenolic acid B is a triterpenoid.
Trametenolic acid has been reported in Laetiporus versisporus, Pappia fissilis, and other organisms with data available. |
| Molecular Formula |
C30H48O3
|
|---|---|
| Molecular Weight |
456.71
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| Exact Mass |
456.36
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| CAS # |
24160-36-9
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| PubChem CID |
12309443
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| Appearance |
White to off-white solid powder
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| Density |
1.07±0.1 g/cm3
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| Melting Point |
259-261 ºC
|
| Vapour Pressure |
3.26E-15mmHg at 25°C
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| LogP |
7.543
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| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
3
|
| Rotatable Bond Count |
5
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| Heavy Atom Count |
33
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| Complexity |
862
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| Defined Atom Stereocenter Count |
7
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| SMILES |
CC(=CCC[C@H]([C@H]1CC[C@@]2([C@@]1(CCC3=C2CC[C@@H]4[C@@]3(CC[C@@H](C4(C)C)O)C)C)C)C(=O)O)C
|
| InChi Key |
NBSBUIQBEPROBM-GIICLEHTSA-N
|
| InChi Code |
InChI=1S/C30H48O3/c1-19(2)9-8-10-20(26(32)33)21-13-17-30(7)23-11-12-24-27(3,4)25(31)15-16-28(24,5)22(23)14-18-29(21,30)6/h9,20-21,24-25,31H,8,10-18H2,1-7H3,(H,32,33)/t20-,21-,24+,25+,28-,29-,30+/m1/s1
|
| Chemical Name |
(2R)-2-[(3S,5R,10S,13R,14R,17R)-3-hydroxy-4,4,10,13,14-pentamethyl-2,3,5,6,7,11,12,15,16,17-decahydro-1H-cyclopenta[a]phenanthren-17-yl]-6-methylhept-5-enoic acid
|
| Synonyms |
(+)-trametenolic acid B Trametenolic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~109.48 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.47 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1896 mL | 10.9479 mL | 21.8957 mL | |
| 5 mM | 0.4379 mL | 2.1896 mL | 4.3791 mL | |
| 10 mM | 0.2190 mL | 1.0948 mL | 2.1896 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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