| Size | Price | Stock | Qty |
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| 500mg |
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| Other Sizes |
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| ln Vitro |
Porcine pancreatic α-amylase is competitively inhibited by trans-chalcone, with a Ki of 48 μM[2]. In MCF-7 cells, trans-Chalcone (30.23-98.03 μM; 24 hours) causes cell cycle arrest and apoptosis[1]. Bcl-2, a protein associated to apoptosis, is expressed less when trans-Chalcone (20-80 μM; 24–48 hours) is applied[1]. In a 24-hour period, trans-Chalcone (58.25 μM; 6) exhibits a stronger induction of CIDEA, a stronger inhibition of Bcl-2, and an induction of APAF1 and BAX. MCF-7 cell viability is inhibited by trans-Chalcone (24 hours) (IC20=30.23 μM; IC50=58.25 μM; IC80=98.03 μM). For the MCF-7 and 3T3 cell lines, trans-Chalcone (48 h) had IC50s of 41.53 μM and 48.41 μM, respectively. There is a noticeable cytotoxic action of trans-Chalcone[1].
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| Cell Assay |
Apoptosis Analysis[1]
Cell Types: MCF-7 cell Tested Concentrations: 30.23, 58.25, 98.03 μM Incubation Duration: 24 hrs (hours) Experimental Results: Induced apoptosis of the breast cancer cell line. Cell Cycle Analysis[1] Cell Types: MCF-7 cell Tested Concentrations: 30.23, 58.25, 98.03 μM Incubation Duration: 24 hrs (hours) Experimental Results: Caused cell cycle arrest in G1. Western Blot Analysis[1] Cell Types: MCF-7 cell Tested Concentrations: 20, 40, 80 μM Incubation Duration: 24, 48 hrs (hours) Experimental Results: decreased the expression of the apoptosis-related protein Bcl-2 and induced the expression of the CIDEA gene. There was marked degradation of cyclin D1 at 48 h. RT-PCR[1] Cell Types: MCF- 7 cell Tested Concentrations: 58.25 μM Incubation Duration: 6, 24 hrs (hours) Experimental Results: Had greater inhibition of Bcl-2, induction of APAF1 and BAX, and strong induction of CIDEA in 24 hrs (hours). |
| References |
[1]. Luis Felipe Buso Bortolotto, et al. Cytotoxicity of trans-chalcone and licochalcone A against breast cancer cells is due to apoptosis induction and cell cycle arrest. Biomed Pharmacother. 2017 Jan;85:425-433.
[2]. Mahmoud Najafian, et al. Trans-chalcone: a novel small molecule inhibitor of mammalian alpha-amylase. Mol Biol Rep. 2011 Mar;38(3):1617-20. [3]. Tamires Aparecida Bitencourt, et al. Trans-chalcone and quercetin down-regulate fatty acid synthase gene expression and reduce ergosterol content in the human pathogenic dermatophyte Trichophyton rubrum. BMC Complement Altern Med. 2013 Sep 17;13:229. |
| Molecular Formula |
C15H12O
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|---|---|
| Molecular Weight |
208.26
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| CAS # |
614-47-1
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| Related CAS # |
Chalcone;94-41-7
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| Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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| SMILES |
O=C(/C(/[H])=C(\[H])/C1C([H])=C([H])C([H])=C([H])C=1[H])C1C([H])=C([H])C([H])=C([H])C=1[H]
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : 100 mg/mL (480.17 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (12.00 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (12.00 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (12.00 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.8017 mL | 24.0085 mL | 48.0169 mL | |
| 5 mM | 0.9603 mL | 4.8017 mL | 9.6034 mL | |
| 10 mM | 0.4802 mL | 2.4008 mL | 4.8017 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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