Size | Price | Stock | Qty |
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25mg |
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50mg |
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100mg |
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250mg |
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500mg |
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1g |
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2g |
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Other Sizes |
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Purity: ≥98%
Trans-Zeatin is a cytokinin/plant hormone that is crucial for cell growth, differentiation, and division.It also encourages the development of lateral buds, seeds, and seedlings, as well as cell division. A plant cytokinin is called trans-Zeatin. Additionally, Trans-Zeatin prevents UV-induced MEK/ERK activation.
Targets |
MMP-1; ERK; JNK; p38 MAPK
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ln Vitro |
Trans-Zeatin pretreatment significantly reduces the amount of UVB-induced MMP-1 expression and c-Jun activation in cultured human skin fibroblasts. This effect may be due to the inhibition of the ERK, JNK, and p38 MAPK signaling pathways[1].
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Cell Assay |
In a 96-well plate, HSFs are sown. Cells are cultured in 100 μl of 10% DMEM in each well for the specified amount of time after receiving varying doses of trans-Zeatin or UVB treatment. The cells are then incubated for 4 hours at 37 °C with 10 μl of MTT solution at a concentration of 5 mg/ml in each well. After removing the medium, each well receives 200 μl of DMSO, which is then pipetted up and down repeatedly to dissolve the formazan crystals. A spectrophotometer is used to measure the absorbance at a wavelength of 570 nm on an ELISA plate reader.
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References |
Molecular Formula |
C10H13N5O
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Molecular Weight |
219.2431
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Exact Mass |
219.11
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Elemental Analysis |
C, 54.78; H, 5.98; N, 31.94; O, 7.30
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CAS # |
1637-39-4
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Related CAS # |
trans-Zeatin-d5;72963-19-0
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Appearance |
Solid powder
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SMILES |
C/C(=C\CNC1=NC=NC2=C1NC=N2)/CO
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InChi Key |
UZKQTCBAMSWPJD-FARCUNLSSA-N
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InChi Code |
InChI=1S/C10H13N5O/c1-7(4-16)2-3-11-9-8-10(13-5-12-8)15-6-14-9/h2,5-6,16H,3-4H2,1H3,(H2,11,12,13,14,15)/b7-2+
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Chemical Name |
(E)-2-methyl-4-(7H-purin-6-ylamino)but-2-en-1-ol
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Synonyms |
Trans Zeatin; (E)-Zeatin; Zeatine; Zeatin
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO: 25~43 mg/mL (114.0~196.1 mM)
Ethanol: ~2 mg/mL (~9.1 mM) |
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.25 mg/mL (5.70 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.25 mg/mL (5.70 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 1.25 mg/mL (5.70 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 4.5612 mL | 22.8061 mL | 45.6121 mL | |
5 mM | 0.9122 mL | 4.5612 mL | 9.1224 mL | |
10 mM | 0.4561 mL | 2.2806 mL | 4.5612 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.