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5g |
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10g |
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25g |
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50g |
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100g |
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200g |
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Other Sizes |
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Purity: ≥98%
Trimethoprim (TMP), an approved drug, is a bacteriostatic antibiotic used mainly in the prophylaxis and treatment of UTIs-urinary tract infectionssuch as bladder infections. Other uses include for middle ear infections and travelers' diarrhea. With sulfamethoxazole or dapsone it may be used for Pneumocystis pneumonia in people with HIV/AIDS. It is taken by mouth. Trimethoprim was first used in 1962.
ln Vitro |
By preventing dihydrofolate (DHFR) from converting dihydrofolate to tetrahydrofolate (THF), trimethoprim prevents the body from getting folate support [1]. In E. coli, trimethoprim (3 μg/mL; 1 hour) causes significant heat shock proteins (Hsps) and protein aggregation. coli cells, indicating that protein misfolding is brought on by trimethoprim sulfate [1]. Induces the DnaK, DnaJ, GroEL, ClpB, and IbpA/B Hsps of E. coli (1.5–3 μg/mL; 1 h). coli, and the stimulated cells are subjected to heat and folic acid [1].
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ln Vivo |
Trimethoprim (10 mg/kg; intravenously; every 12 hours; 3 days) demonstrated antimicrobial activity against H. Protective effect against meningococcal infections, Streptococcus pneumoniae, influenza bacteria, and the like [2]. It can bind to thiomaltose (TM-TMP) and show stability, with an approximate half-life of one hour in complete serum and an approximate MIC value of one micromicrogram against E. coli [2].
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Animal Protocol |
Animal/Disease Models: Female C3H/HeOuJ mouse (transurethral infection containing 1-2 × 107 in 50 μL suspension of 3% E. coli CFU in isoflurane) [2]
Doses: 10 mg/kg Route of Administration: intravenous (iv) (iv)injection; once every 12 hrs (hrs (hours)); 3 d Experimental Results: It has antibacterial activity against Haemophilus influenzae, Streptococcus pneumoniae, Escherichia coli and Neisseria meningitidis. The CD50 of infected patients is 150 mg respectively. /kg, 335 mg/kg, 27.5 mg/kg and 8.4 mg/kg mice. Animal/Disease Models: Fertilized eggs (H3N2 virus is injected into the amniotic membrane and allantoic cavity on day 8) [4] Doses: 10 mg/mL; 0.5 mL Route of Administration: Trimethoprim-zinc composite suspension is injected into the air sac; single dose Experimental Results: The virus titer was diminished and the survival rate of chicken embryos was improved. Survival rates peaked at a ratio of approximately 0.18 (Zn/trimethoprim). |
References |
[1]. Laskowska, E., et al., Trimethoprim induces heat shock proteins and protein aggregation in E. coli cells. Curr Microbiol, 2003. 47(4): p. 286-9.
[2]. Brogden, R.N., et al., Trimethoprim: a review of its antibacterial activity, pharmacokinetics and therapeutic use in urinary tract infections. Drugs, 1982. 23(6): p. 405-30. [3]. Xiaojian Wang, et al. A Trimethoprim Conjugate of Thiomaltose Has Enhanced Antibacterial Efficacy In Vivo. Bioconjug Chem. 2018 May 16;29(5):1729-1735. [4]. El Habbal MH. Combination therapy of zinc and trimethoprim inhibits infection of influenza A virus in chick embryo. Virol J. 2021 Jun 3;18(1):113. |
Molecular Formula |
C14H18N4O3
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Molecular Weight |
290.32
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CAS # |
738-70-5
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Related CAS # |
Trimethoprim lactate;23256-42-0;Trimethoprim-d9;1189460-62-5;Trimethoprim-d3;1189923-38-3;Trimethoprim sulfate;56585-33-2;Trimethoprim hydrochloride;60834-30-2;Trimethoprim-13C3;1189970-95-3
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
NC1=NC=C(CC2=CC(OC)=C(OC)C(OC)=C2)C(N)=N1
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InChi Key |
IEDVJHCEMCRBQM-UHFFFAOYSA-N
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InChi Code |
InChI=1S/C14H18N4O3/c1-19-10-5-8(6-11(20-2)12(10)21-3)4-9-7-17-14(16)18-13(9)15/h5-7H,4H2,1-3H3,(H4,15,16,17,18)
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Chemical Name |
5-(3,4,5-Trimethoxybenzyl)pyrimidine-2,4-diamine
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Synonyms |
Proloprim; Trimethoprim; Trimpex, Monotrim, Triprim among others
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~50 mg/mL (~172.22 mM)
H2O : ~0.67 mg/mL (~2.31 mM) |
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (8.61 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (8.61 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (8.61 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 3.4445 mL | 17.2224 mL | 34.4448 mL | |
5 mM | 0.6889 mL | 3.4445 mL | 6.8890 mL | |
10 mM | 0.3444 mL | 1.7222 mL | 3.4445 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.