| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| Other Sizes |
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| Enzyme Assay |
Auto-acetylation reactions of full-length p300 were performed in lysine acetyltransferase assay buffer at 30°C for 10 min with or without TTK21, followed by the addition of 1 μl of 4.7 Ci/mmol [3H]acetyl-CoA (NEN–PerkinElmer). The reaction mixture was further incubated for another 10 min at 30°C. The 3H-labeled acetylated p300 was visualized by fluorography followed by autoradiography.[1]
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|---|---|
| Cell Assay |
One equivalent of SOCl2 diluted in DCM was added dropwise to a suspension of 100 mg of CSP in DCM, followed by the addition of few drops of DMF . The reaction mixture was stirred at room temperature for 8–9 h. TTK21 dissolved in DCM was added dropwise to this solution. The reaction mixture was stirred for 8–9 h at room temperature. The solvent was then evaporated and washed with cold water. The crude product was centrifuged and the supernatant (i.e., water) was removed; this procedure was repeated 7–8 times. Washing was then performed using DCM and the supernatant was subsequently tested for absence of TTK21. The CSP-TTK21 conjugated was then dried at 60°C for 2–3 d.[1]
Highly purified HeLa core histones were incubated in HAT assay buffer at 30°C for 10 min with or without baculovirus-expressed recombinant p300 or CBP in the presence or absence of TTK21, followed by addition of 1 μl of 3.6 Ci/mmol [3H]acetyl-CoA (NEN–PerkinElmer) and incubated for 10 min in a final volume of 30 μl at 30°C.[1] |
| References |
|
| Molecular Formula |
C17H15CLF3NO2
|
|---|---|
| Molecular Weight |
357.7572
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| Exact Mass |
357.074
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| Elemental Analysis |
C, 57.07; H, 4.23; Cl, 9.91; F, 15.93; N, 3.92; O, 8.94
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| CAS # |
709676-56-2
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| PubChem CID |
68453302
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| Appearance |
White to off-white solid powder
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| Density |
1.3±0.1 g/cm3
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| Boiling Point |
369.8±42.0 °C at 760 mmHg
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| Flash Point |
177.4±27.9 °C
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| Vapour Pressure |
0.0±0.8 mmHg at 25°C
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| Index of Refraction |
1.553
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| LogP |
5.7
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
24
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| Complexity |
419
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C(NC1=CC=C(Cl)C(C(F)(F)F)=C1)(=O)C1=CC=CC=C1OCCC
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| InChi Key |
AQJBXYBDNZHZRE-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C17H15ClF3NO2/c1-2-9-24-15-6-4-3-5-12(15)16(23)22-11-7-8-14(18)13(10-11)17(19,20)21/h3-8,10H,2,9H2,1H3,(H,22,23)
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| Chemical Name |
N-[4-chloro-3-(trifluoromethyl)phenyl]-2-propoxybenzamide
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| Synonyms |
TTK-21; TTK21; TTK 21;
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~279.52 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (6.99 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7952 mL | 13.9758 mL | 27.9517 mL | |
| 5 mM | 0.5590 mL | 2.7952 mL | 5.5903 mL | |
| 10 mM | 0.2795 mL | 1.3976 mL | 2.7952 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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