Size | Price | Stock | Qty |
---|---|---|---|
5mg |
|
||
10mg |
|
||
25mg |
|
||
50mg |
|
||
100mg |
|
||
250mg |
|
||
500mg |
|
||
Other Sizes |
|
Purity: ≥98%
Tubercidin (7-Deazaadenosine), an antibiotic isolated from Streptomyces tubercidicus, is a DNA/RNA Synthesis inhibitor. Tubercidin is a toxic adenosine analogue with antiviral, antitrypanosomal, and antifungal functions. It inhibits multiple metabolic processes, including RNA processing, nucleic acid synthesis, protein synthesis, and methylation of tRNA through intracellular incorporation into nucleic acids. Tubercidin acts as a plant antifungal, inhibits mammalian SAH hydrolase (SAHH), and blocks purine biosynthesis in Candida famata.
ln Vitro |
The inhibitory impact of tubercidin (7-Deazaadenosine) (0-10 nM; 14 d) on erythroid human myeloid progenitor cells and bone marrow is dose-dependent. On CFU-GM and BFU-E cells, tubercidin's half-life (IC50) is 3.4 nM and 3.7 nM, respectively.
|
---|---|
ln Vivo |
In order to preserve the lethality of shell Tubercidin and enable a second iteration of the regimen, finally with 100%, Tubercidin (7-Deazaadenosine) (i.p. ; 5 mg/kg; 10 days) collaborates with NBMPR-P [3].
|
Cell Assay |
Cytotoxicity assay [2]
Cell Types: human bone marrow progenitor cells Tested Concentrations: 0-10nM Incubation Duration: 14 days Experimental Results: Dose-dependent inhibitory effect on CFU-GM and BFU-E. |
Animal Protocol |
Animal/Disease Models: Female CD1 mice[2]
Doses: 5 mg/kg Route of Administration: intraperitoneal (ip) injection; ]. 5 mg/kg; 10 day Experimental Results: Protect mice from tuberculin. |
References |
[1]. Bloch A, et al. On the mode of action of 7-deaza-adenosine (Tubercidin). Biochim Biophys Acta. 1967 Mar 29;138(1):10-25.
[2]. Bergstrom DE, et al. Antiviral activity of C-5 substituted tubercidin analogues. J Med Chem. 1984 Mar;27(3):285-92. [3]. Kouni MH, et al. Prevention of tubercidin host toxicity by nitrobenzylthioinosine 5'-monophosphate for the treatment of schistosomiasis. Antimicrob Agents Chemother. 1989 Jun;33(6):824-7. |
Molecular Formula |
C11H14N4O4
|
---|---|
Molecular Weight |
266.25326
|
CAS # |
69-33-0
|
Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
|
SMILES |
NC1=NC=NC2=C1C=CN2[C@H]3[C@H](O)[C@H](O)[C@@H](CO)O3
|
Chemical Name |
(2R,3R,4S,5R)-2-(4-amino-7H-pyrrolo[2,3-d]pyrimidin-7-yl)-5-(hydroxymethyl)tetrahydrofuran-3,4-diol
|
Synonyms |
7-Deazaadenosine Antibiotic XK 101-1 B 120121 NSC 56408 SKI 26996
Sparsomycin A TBC Tubercidin Tubercidine U 10071 U-10071 U10071
|
HS Tariff Code |
2934.99.9001
|
Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
Solubility (In Vitro) |
DMSO : ≥ 30 mg/mL (~112.68 mM)
|
---|---|
Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (9.39 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (9.39 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (9.39 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 3.7559 mL | 18.7793 mL | 37.5587 mL | |
5 mM | 0.7512 mL | 3.7559 mL | 7.5117 mL | |
10 mM | 0.3756 mL | 1.8779 mL | 3.7559 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.