| Size | Price | Stock | Qty |
|---|---|---|---|
| 500mg |
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| 1g |
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| Other Sizes |
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| ln Vitro |
Uric acid (400 μM; 48 hours) protects Caco-2 cells from indomethacin-induced lipid peroxidation [2]. Co-treatment of cells with indomethacin and uric acid (200 μM IND plus 400 μM UA; 24 hours) significantly lowered ROS levels compared to cells treated with indomethacin alone. Cell viability in Caco-2 cells treated with both indomethacin and uric acid (200 μM IND plus 400 μM UA; 24 h) was higher than that in cells treated with indomethacin alone. Uric acid has a protective effect against indomethacin-induced alterations in intestinal cells through its antioxidant activity [2].
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|---|---|
| Animal Protocol |
Animal/Disease Models: 8weeks old male C57BL/6J mice [2]
Doses: 250 mg/kg body weight Route of Administration: Po Experimental Results: When mice are given indomethacin at the same time, uric acid can be used for animal modeling to construct a hypertension model. . After oral administration of uric acid, the ulcer area was Dramatically diminished in a uric acid dose-dependent manner. |
| References |
[1]. Wang Q, et al. Recent Progress on Uric Acid Detection: A Review. Crit Rev Anal Chem. 2020;50(4):359-375.
[2]. Yasutake Y, et al. Uric acid ameliorates indomethacin-induced enteropathy in mice through its antioxidant activity. J Gastroenterol Hepatol. 2017 Nov;32(11):1839-1845. |
| Molecular Formula |
C5H4N4O3
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|---|---|
| Molecular Weight |
168.1103
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| CAS # |
69-93-2
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| Related CAS # |
Uric acid sodium;1198-77-2;Uric acid-13C,15N3;2421217-23-2;Uric acid-15N2;62948-75-8;Uric acid-13C3;2832998-22-6
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| Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
|
| SMILES |
O=C1C2=C(N([H])C(N1[H])=O)N([H])C(N2[H])=O
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
1M NaOH : 8.33 mg/mL (~49.55 mM)
H2O : ~6.25 mg/mL (~37.18 mM) DMSO :< 1 mg/mL |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 10 mg/mL (59.48 mM) in 0.5% CMC-Na/saline water (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.9485 mL | 29.7424 mL | 59.4849 mL | |
| 5 mM | 1.1897 mL | 5.9485 mL | 11.8970 mL | |
| 10 mM | 0.5948 mL | 2.9742 mL | 5.9485 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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