| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| Other Sizes |
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| ln Vitro |
VP-4604 (2.5 mM, 18 hours) inhibits Pseudomonas aeruginosa and drug-resistant Staphylococcus aureus with growth inhibition (%) GI >95% [1]. With a CC50 value of >118.4 mM for human embryonic kidney (HEK-293) ATCC CRL-1573 and an HC10 value of >118.4 μM for human red blood cells (RBC), VP-4604 demonstrates negligible cytotoxicity [1]. Mammalian cells, such as HaCaT and lymphocytes, can withstand VP-4604 at a concentration of 2.5 mM, with growth inhibition rates (%) of 44.1% and >50%, respectively [1].
|
|---|---|
| Cell Assay |
Cell proliferation experiment [1]
Cell Types: S. aureus ATCC25923, P. aeruginosa ATCC9027 Tested Concentrations: 0.625 mM, 1.25 mM, 2.5 mM Incubation Duration: 18 hrs (hours) Experimental Results: Shows antibacterial effect. Cytotoxicity assay[1] Cell Types: HaCaT line (human keratinocytes), lymphocytes (from healthy adult peripheral blood) Tested Concentrations: 0, 1, 10, 100, 1000, 2500 μM Incubation Duration: 48 or 72 hrs (hours) Experimental Results: Displays low toxicity to mammalian cells. |
| References |
| Molecular Formula |
C11H14N2O4S
|
|---|---|
| Molecular Weight |
270.30486
|
| Exact Mass |
270.067
|
| CAS # |
64268-93-5
|
| PubChem CID |
723624
|
| Appearance |
White to off-white solid powder
|
| Density |
1.374g/cm3
|
| Boiling Point |
435.9ºC at 760 mmHg
|
| Melting Point |
166ºC
|
| Flash Point |
217.4ºC
|
| Index of Refraction |
1.592
|
| LogP |
3.311
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
5
|
| Rotatable Bond Count |
2
|
| Heavy Atom Count |
18
|
| Complexity |
384
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
XDPAFNJUKBYWBZ-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C11H14N2O4S/c14-13(15)10-4-6-11(7-5-10)18(16,17)12-8-2-1-3-9-12/h4-7H,1-3,8-9H2
|
| Chemical Name |
1-(4-nitrophenyl)sulfonylpiperidine
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~25 mg/mL (~92.49 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (9.25 mM) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.6996 mL | 18.4980 mL | 36.9959 mL | |
| 5 mM | 0.7399 mL | 3.6996 mL | 7.3992 mL | |
| 10 mM | 0.3700 mL | 1.8498 mL | 3.6996 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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