Size | Price | Stock | Qty |
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10mg |
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25mg |
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50mg |
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100mg |
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250mg |
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500mg |
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1g |
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Other Sizes |
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WST-3 is a novel and highly sensitive tetrazolium reagent that is able to produce a water-soluble, colored formazan dye with reducing agents such as NADH.
References |
: Wawra A, Friesl-Hanl W, Jäger A, Puschenreiter M, Soja G, Reichenauer T, Watzinger A. Investigations of microbial degradation of polycyclic aromatic hydrocarbons based on (13)C-labeled phenanthrene in a soil co-contaminated with trace elements using a plant assisted approach. Environ Sci Pollut Res Int. 2018 Mar;25(7):6364-6377.
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Molecular Formula |
C19H10IN6NAO10S2
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Molecular Weight |
696.3411
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CAS # |
515111-36-1
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
O=S(C1=CC=C(C2=NN(C3=CC=C(I)C=C3)[N+](C4=CC=C([N+]([O-])=O)C=C4[N+]([O-])=O)=N2)C(S(=O)([O-])=O)=C1)([O-])=O.[Na+]
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InChi Key |
LKVNWZJLLRMMAS-UHFFFAOYSA-M
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InChi Code |
InChI=1S/C19H11IN6O10S2.Na/c20-11-1-3-12(4-2-11)23-21-19(15-7-6-14(37(31,32)33)10-18(15)38(34,35)36)22-24(23)16-8-5-13(25(27)28)9-17(16)26(29)30/h1-10H,(H-,31,32,33,34,35,36)/q+1/p-1
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Chemical Name |
Sodium 4-[2-(2,4-dinitrophenyl)-3-(4-iodophenyl)tetrazol-2-ium-5-yl]benzene-1,3-disulfonate
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Synonyms |
WST-3 WST 3 WST3
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~100 mg/mL (~143.61 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (3.59 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 1.4361 mL | 7.1804 mL | 14.3608 mL | |
5 mM | 0.2872 mL | 1.4361 mL | 2.8722 mL | |
10 mM | 0.1436 mL | 0.7180 mL | 1.4361 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.