Size | Price | Stock | Qty |
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10mg |
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25mg |
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50mg |
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100mg |
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250mg |
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Other Sizes |
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ln Vitro |
The EC50 value of YTX-465 (1-10000 nM) for the rescue of alpha-synuclein (α-syn) toxicity is 0.013 μM [1]. 0.05 μM; 0–2 days) of YTX-465 recovers the growth of yeast expressing α-Syn [1]. In wild-type yeast producing OLE1, YTX-465 (0, 10, 40, 160, 640, 2500 nM; 4h) increases Ole1 protein levels in a concentration-dependent manner, indicating that YTX-465 generates a negative feedback loop [1]. Fat desaturation in wild-type yeast is lowered by YTX-465 (0, 0.03, 0.09, 0.27, 0.81 μM; 6 hours) in a concentration-dependent manner, with a 50% reduction at 0.03 μM [1]. In yeast of the wild type, YTX-465 (0.25 μM; 8 hours) decreases the desaturation index (DI) of all main classes of membrane phospholipids [1].
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References |
[1]. Vincent BM, et al. Inhibiting Stearoyl-CoA Desaturase Ameliorates α-Synuclein Cytotoxicity. Cell Rep. 2018 Dec 4;25(10):2742-2754.e31.
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Molecular Formula |
C25H26N6O3
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Molecular Weight |
458.522
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Exact Mass |
458.2066
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Elemental Analysis |
C, 65.49; H, 5.72; N, 18.33; O, 10.47
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CAS # |
2225824-53-1
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Appearance |
Solid powder
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SMILES |
O1C(C2CCN(C(CNC(C3C=CC=CC=3)=O)=O)CC2)=NC(C2=CC=C3C(C)=NN(C)C3=C2)=N1
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Synonyms |
YTX 465; YTX-465; YTX465;
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~100 mg/mL (~218.10 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.45 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (5.45 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (5.45 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.1809 mL | 10.9046 mL | 21.8093 mL | |
5 mM | 0.4362 mL | 2.1809 mL | 4.3619 mL | |
10 mM | 0.2181 mL | 1.0905 mL | 2.1809 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
YTX-465 Reduces Lipid Desaturation, and Its Effects Are Reversed by Adding Ole1 Products. J Gen Virol. 2013, 94(Pt 12), 2710-2716. td> |
Reducing Lipid Desaturation with YTX-465 Reduces α-Syn Aggregation and Vesicle Trafficking Defects. J Gen Virol. 2013, 94(Pt 12), 2710-2716. td> |