RNA synthesis, which is also called DNA transcription, is a highly selective process.Beyond RNA synthesis, RNA polymerase II transcription plays a more active role in the maturation, monitoring, and export of mRNA to the cytoplasm.
In order to fully repair the DNA after a single-strand break, DNA ligase constructs the final phosphodiester bond using the complementary strand of the double helix as a template.Substrates with RNA strands or mismatched base pairs near the ends of the nicked DNA are discriminated against by DNA ligases. Bleomycin (BLM) is a S-independent radiomimetic agent that damages DNA by creating free radicals that attack the C-4′ position in the deoxyribose backbone. This causes the ribose ring to open and strand breaks to occur.
M-MuLV Reverse Transcriptase (RNase H) and random hexamer primers are used to create first strand cDNA. The next step involves the use of DNA Polymerase I and RNase H to create second strand cDNA. Exonuclease/polymerase activity transforms the remaining overhangs into blunt ends. DNA fragments' 3′ ends are adenylated before being ligated with a NEBNext Adaptor with a hairpin loop structure to get the samples ready for hybridization. The two pathways that BET bromodomain inhibition most commonly affects are cell cycle and DNA replication. The translation of mRNA into proteins is inhibited by cycloheximide.
Structure | Cat No. | Product Name | CAS No. | Product Description |
---|---|---|---|---|
V38155 | RP-6685 | 2832047-80-8 | RP-6685 is a novel, orally active inhibitor of DNA polymerase theta (Polθ) with an IC50 of 5.8 nM (PicoGreen assay). | |
V55122 | rU Phosphoramidite (DMT-2'O-TBDMS-rU phosphoramidite) | 118362-03-1 | rU Phosphoramidite is a phosphoramidite monomer that may be utilized in the preparation /synthesis of oligonucleotides. | |
V37900 | S-(N-PhenethylthiocarbaMoyl)-L-cysteine (PEITC-Cys) | 53330-02-2 | S-(N-PhenethylthiocarbaMoyl)-L-cysteine (PEITC-Cys)is an antileukemia agent that also has anticarcinogenic properties. | |
V35273 | S-MGB-234 | 1970223-53-0 | S-MGB-234 is a minor groove adhesive for African animal trypanosomiasis (AAT). | |
V1434 | SCR7 | 1533426-72-0 | SCR7 (SCR-7; SCR 7) is a potent and selective inhibitor of DNA Ligase IV with potential anticancer activity. | |
V1477 | Sofosbuvir (PSI-7977; GS-7977) | 1190307-88-0 | Sofosbuvir (also known as PSI-7977, GS-7977; PSI7977; GS7977; Vosevi; Hepcinat; Hepcvir; Resof; Sovaldi and Virunon), ablockbuster anti-HCV drug, is an HCV NS5B polymerase inhibitor that has been approved for the treatment of chronic hepatitis C virus (HCV) infections. | |
V15074 | Sorivudine | 77181-69-2 | Sorivudine (BV-araU; Bravavir; ARYS-01; SQ-32756; BVAU; JA-001; YN-72; DRU-0136) is a novel and potent DNA polymerase inhibitor with the potential to be used for the treatment of herpes zoster (shingles). | |
V75892 | SP-471P | 2768011-36-3 | SP-471P is a potent Dengo virus (DENV) protease inhibitor (antagonist) with EC50s of 5.9 μM, 1.4 μM, 5.1 μM and 1.7 μM for DENV1, DENV2, DENV3 and DENV4 respectively, and CC50 is greater than 100 μM. | |
V55192 | Spacer phosphoramidite C3 | 110894-23-0 | Spacer phosphoramidite C3 is a phosphoramidite monomer that may be utilized in the preparation /synthesis of oligonucleotides. | |
V81432 | SRE-II | SRE-II, an amide analogue, is an activatable photosensitizer with reduced fluorescence and photosensitizing capabilities for use in photodynamic cancer research. | ||
V1856 | Streptozotocin (STZ) | 18883-66-4 | Streptozotocin (also known as NSC-85998, STZ, Streptozotocin; U 9889) is a glucosamine-nitrosourea derivative and a potent DNA-methylating agent with IC50s of 11.7, 904 and 1024 μg/mL in HL60, K562 and C1498 cells respectively. | |
V35240 | Synucleozid hydrochloride (NSC 377363 hydrochloride) | 2741856-68-6 | Synucleozid (NSC 377363) is a novel inhibitor of SNCA mRNA encoding alpha-synuclein. | |
V35272 | T-2513 | 288247-87-0 | T-2513 is a potent topoisomerase I inhibitor. | |
V35271 | T-2513 hydrochloride | 187793-52-8 | T-2513 hydrochloride is a potent topoisomerase I inhibitor. | |
V35232 | T2AA | 1380782-27-3 | T2AA is a monoubiquitinated proliferating cell nuclear antigen (PCNA) inhibitor that induces G1 cell cycle arrest, increases the formation of double-strand breaks (DSBs), and inhibits DNA repair. | |
V81495 | T4 RNA ligase | T4 RNA ligase is an RNA ligase that catalyzes the ligation of single-stranded DNA molecules. | ||
V81496 | T4 UvsX Recombinase | T4 UvsX Recombinase helps initiate DNA replication on double-stranded DNA templates by catalyzing the synapse between the template and a homologous DNA single strand that serves as a primer. | ||
V55150 | T7 RNA polymerase | 9014-24-8 | T7 RNA polymerase is a polymerase that is produced by Escherichia coli and is derived from the T7 phage RNA polymerase gene. | |
V55133 | Taq DNA polymerase | 2304873-37-6 | Taq DNA polymerase is a thermostable DNA polymerase that may be utilized in PCR. | |
V13524 | TAS-102 (Trifluridine/Tipiracil HCl) | 733030-01-8 | TAS-102 (a mixture of Trifluridine and tipiracil hydrochloridein a 1:0.5 molar ratio) is a combination drug under investigation for treating metastatic colorectal cancer. |