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DNA(RNA) Synthesis

DNA(RNA) Synthesis

RNA synthesis, which is also called DNA transcription, is a highly selective process.Beyond RNA synthesis, RNA polymerase II transcription plays a more active role in the maturation, monitoring, and export of mRNA to the cytoplasm.

In order to fully repair the DNA after a single-strand break, DNA ligase constructs the final phosphodiester bond using the complementary strand of the double helix as a template.Substrates with RNA strands or mismatched base pairs near the ends of the nicked DNA are discriminated against by DNA ligases. Bleomycin (BLM) is a S-independent radiomimetic agent that damages DNA by creating free radicals that attack the C-4′ position in the deoxyribose backbone. This causes the ribose ring to open and strand breaks to occur.

M-MuLV Reverse Transcriptase (RNase H) and random hexamer primers are used to create first strand cDNA. The next step involves the use of DNA Polymerase I and RNase H to create second strand cDNA. Exonuclease/polymerase activity transforms the remaining overhangs into blunt ends. DNA fragments' 3′ ends are adenylated before being ligated with a NEBNext Adaptor with a hairpin loop structure to get the samples ready for hybridization. The two pathways that BET bromodomain inhibition most commonly affects are cell cycle and DNA replication. The translation of mRNA into proteins is inhibited by cycloheximide.

DNA(RNA) Synthesis related products

Structure Cat No. Product Name CAS No. Product Description
(+)-TK216 V52845 (+)-TK216 1903783-77-6 (+)-TK216 is the enantiomer of TK216.
(-)-TK216 V52847 (-)-TK216 1903783-78-7 (-)-TK216 is the enantiomer of TK216 (HY-122903).
(E/Z)-NSAH V55140 (E/Z)-NSAH 54009-54-0 (E/Z)-NSAH is an isomer of NSAH (HY-114503).
(±)2-(14,15-Epoxyeicosatrienoyl) glycerol V104053 (±)2-(14,15-Epoxyeicosatrienoyl) glycerol 848667-56-1 (±)2-(14,15-Epoxyeicosatrienoyl)glycerol ((±)2-14,15-EG) is a novel CYP450 metabolite of 2-arachidonoylglycerol (2-AG) in the kidney.
1,4-Anthraquinone V52527 1,4-Anthraquinone 635-12-1 1,4-Anthraquinone is a novel anticancer agent.
1,7-Heptanediamine V96929 1,7-Heptanediamine 646-19-5 1,7-Heptanediamine (C7) is a deoxyalkali synthase inhibitor with IC50 of 4.5 μM.
1-Hydroxyanthraquinone (1-羟基蒽醌) V53307 1-Hydroxyanthraquinone 129-43-1 1-Hydroxyanthraquinone is an orally bioactive naturally occurring compound developed from some plants like Tabebuia avellanedae and has carcinogenic effects.
1-p-Chlorobenzyl-1,2,3-triazole-5′-O-DMT-dU Phosphoramidite V77531 1-p-Chlorobenzyl-1,2,3-triazole-5′-O-DMT-dU Phosphoramidite 1-p-Chlorobenzyl-1,2,3-triazole-5′-O-DMT-dU Phosphoramidite is a phosphoramidite monomer that may be utilized in the preparation /synthesis of oligonucleotides.
12(S)-HETE V84362 12(S)-HETE 54397-83-0
16,16-Dimethyl prostaglandin A1 (di-Me-PGA1) V83605 16,16-Dimethyl prostaglandin A1 (di-Me-PGA1) 41692-24-4
2'-Deoxy-2'-fluoroadenosine 5'-triphosphate tetralithium (AfTP (tetralithium), 2'-Fluoro-2'-deoxy-ATP (tetralithium)) V77574 2'-Deoxy-2'-fluoroadenosine 5'-triphosphate tetralithium (AfTP (tetralithium), 2'-Fluoro-2'-deoxy-ATP (tetralithium)) 2'-Deoxy-2'-fluoroadenosine 5'-triphosphate tetralithium is an ATP analogue that also has a strong inhibitory effect on the synthesis of poly(AU).
2'-Deoxy-5'-O-DMT-N4-Fmoc-5-methylcytidine 3'-CE-phosphoramidite V55148 2'-Deoxy-5'-O-DMT-N4-Fmoc-5-methylcytidine 3'-CE-phosphoramidite 176755-87-6 2'-Deoxy-5'-O-DMT-N4-Fmoc-5-methylcytidine 3'-CE-phosphoramidite is an analogue of the nucleoside cytosine.
2'-Deoxy-5'-O-DMT-N6-Fmoc-adenosine 3'CE-phosphoramidite V55158 2'-Deoxy-5'-O-DMT-N6-Fmoc-adenosine 3'CE-phosphoramidite 109420-86-2 2'-Deoxy-5'-O-DMT-N6-Fmoc-adenosine 3'CE-phosphoramidite is an analog of an adenine nucleoside.
2'-Deoxyadenosine 5'-di-phos-phate disodium V106552 2'-Deoxyadenosine 5'-di-phos-phate disodium 72003-83-9 2'-Deoxyadenosine 5'-diphosphate disodium (dADP disodium) is an inhibitor of bacterial polyadenylate polymerase and can be used to synthesize deoxyadenosine oligonucleotides with enzymes such as polynucleotide phosphorylase of Escherichia coli.
2'-Deoxyguanosine 5'-monophosphate disodium hydrate V89929 2'-Deoxyguanosine 5'-monophosphate disodium hydrate 146877-98-7 2'-Deoxyguanosine 5'-monophosphate (5'-Deoxyguanylic acid; dGMP) disodium hydrate is an oxidizable target of the photosensitizer pterin (PT) and can be used to evaluate the photosensitizing properties of biopterins (such as Bip, Fop, and Cap).
2'-Deoxyguanosine-(N-iBu)-3'-methyl-phosphoramidite V55184 2'-Deoxyguanosine-(N-iBu)-3'-methyl-phosphoramidite 84416-84-2 2'-Deoxyguanosine-(N-iBu)-3'-methyl-phosphoramidite is a phosphoramidite monomer that may be utilized in the preparation /synthesis of oligonucleotides.
2′-O-(2-Methoxyethyl)adenosine V52985 2′-O-(2-Methoxyethyl)adenosine 168427-74-5 2′-O-(2-Methoxyethyl)adenosine is a compound useful in the preparation /synthesis of oligonucleotides.
2'-O-methyladenosine 5'-phosphate V103755 2'-O-methyladenosine 5'-phosphate 24121-00-4 2'-O-methyladenosine 5'-phosphate is a ribosomal nucleotide complexing group, mainly used for DNA coupling.
2′-O-Methylguanosine 5′-monophosphate V103750 2′-O-Methylguanosine 5′-monophosphate 24121-01-5 2′-O-methylguanosine 5′-monophosphate is a ribosomal nucleotide complexing group (pGm) that is primarily used for DNA conjugation.
2'-O-MOE-5MeU-3'-phosphoramidite (2'-O-MOE-5-ME-U 亚磷酰胺单体) V55168 2'-O-MOE-5MeU-3'-phosphoramidite (2'-O-MOE-5-ME-U phosphoramidite monomer) 163878-63-5 2'-O-MOE-5MeU-3'-phosphoramidite is a phosphoramidite monomer that may be utilized in the preparation /synthesis of oligonucleotides.
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